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Calcium Magnesium Buffer for overcoming chelation effect




Endotoxin-free magnesium buffer for overcoming the interference by chelation effect

1. Product information

Limulus amoebocyte lysate endotoxin assay for detection of bacterial endotoxins LAL lysate and endotoxin reaction require magnesium and calcium ions. Test samples that have a high affinity for divalent cations will chelate or sequester them from the LAL lysate formulation. Without the proper concentration of divalent cations, the enzymes in the lysate cannot function properly. The LAL lysate and endotoxin reaction was inhibited. In order to overcome the chelation effect cause LAL interference, a buffer contains calcium and magnesium could be used to dilute the test sample.

2. Product parameter

Endotoxin level < 0.005EU/ml

3. Product features and application

Supply additional magnesium to overcome the LAL and endotoxin reaction inhibition caused by the chelation effect.

4. Product parameter

Catalog   Number

Description

Note

Package

BY10

10mM Magnesium   Buffer, 10ml/vial

Used   to overcome chelation effect.

10   vials/pack

5. Ordering FAQ

Product condition: The LAL reagent sensitivity and the Control Standard Endotoxin potency are assayed against USP Reference Standard Endotoxin. The LAL reagent kits come with product instruction, Certificate of Analysis, MSDS.

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