BIO-lottTMBlotting Membrane
Blotting membranes are used widely in biotechnology, such as protein transfers, western transfers, protein dot or slot blots, traditional DNA and RNA transfers, nucleic acid detection, northerns and southerns. Membrane Solutions affords all kinds of blotting membrane including PVDF transfer membrane, nitrocellulose membrane and nylon membrane. We can custom blotting membrane, like the shape, the size, the contents, the package and different use as per your requirements.
Selecting an blotting membrane from MS®
MS® Life Science offers membranes for use in transfer and immobilization procedures. Selecting the appropriate membrane is critical to the success of a nucleic acid or protein transfer procedure. The several types of immobilon transfer membranes exhibit different performance characteristics which can directly affect the outcome of a specific technique.
NC Blotting Membrane | PVDF Blotting Membrane | |
Composition | 100% Pure Nitrocellulose | Polyvinylidene Fluoride |
Description | One of the most widely used membranes in analytical and research applications. Minimize amounts of wetting agent and have a lower water extractable content. | PVDF membrane has high protein adsorption, so proteins won’t be lost during transfer or re-probing. |
Open pore structure makes accessing bound proteins and removing unbound probes easily. Membranes optimized for fluorescent blots dramatically increase signal for high sensitivity in quantitative, multiplexing applications. | ||
Applications | Nucleic acid and protein transfers. Suitable for Western and Southern blotting. | Western blotting, binding assays, amino acid analysis, N-terminal protein sequencing, dot/slot blotting, glycoprotein visualization, lipopolysaccharide analysis |
Detection Methods | Chromogenic, Radioactive, Fluorescent | Direct Stain, Enzyme-antibody Conjugates, Chromogenic, Chemiluminescent |
Typical protein binding capacity | 125ug/cm2 | |
Compatible stains | Amido black, India ink, Ponceau-S red,Colloidal gold, CPTS | Coomassie brilliant blue, Amido black, India ink, Ponceau-S red, Colloidal gold, CPTS, Toluidine blue, Transillumination, Sypro’s ruby |
Works best for | Colony/Plaque Lifts | Protein Transfers |
Suitable for | Nucleic Acid and Protein | Nucleic Acid |
Advantages | Excellent strength | Chemical resistance |
No support fabric | No discoloration | |
No detergents added | Nonflammable | |
100% pure nitrocellulose | High strength | |
Binding Interaction | Hydrophobic & Electrostatic | Hydrophobic |
Method of Immobilization | UV Crosslink Baking (Vacuum Oven) | Electroblott |
Ordering Information
Item # | Description | Pcs per box | |
NC Blotting Membrane | |||
MSNC02030301 | Pore: 0.22μm, Filter Size: 300*3000mm | 1 | |
MSNC04530301 | Pore: 0.45μm, Filter Size: 300*3000mm | 1 | |
PVDF Blotting Membrane | |||
MSPVDF02030301 | Pore: 0.22μm, Filter Size: 300*3000mm | 1 | |
MSPVDF04530301 | Pore: 0.45μm, Filter Size: 300*3000mm | 1 |